Archives of Acoustics, 44, 2, pp. 357–363, 2019

Separation of Cells From Plasma by Means of Ultrasonics

Andrzej WŁOCH
Rzeszow University of Technology

Henryka CZYŻ
Rzeszow University of Technoogy

Rzeszow University of Technology

This paper presents an analysis of use of ultrasonic standing wave in cell separation from bodily fluids based on the example of erythrocyte separation from plasma. It describes movement of red blood cells in plasma under the influence of the acoustic field (whose forces result from interaction of red blood cells with plasma as the vibrating medium) and under the influence of resistance forces in Stokes’ and Oseen’s approximation. The general properties of solutions of the motion equation are given. The solutions for the parameters of the ultrasonic wave and blood cells which are interesting in terms of practical applications in medical diagnostics are discussed. Time constants of the cell transportation to the regions of stable equilibrium in the field of ultrasonic standing wave are estimated. The formulas which determine the time needed to obtain the assumed concentration increase in plasma in nodes and/or anti-nodes of the standing wave are derived.
Keywords: ultrasound standing wave; medical diagnostics; body fluids; blood; red blood cells; cells separation; plasma
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Barnett S.B. (2000), Biophysical aspects of diagnostic ultrasound, Ultrasound in Medicine and Biology, 26, Suppl. 1, 68–70,

Benes E. et al. (2003), The ultrasonic h-shape separator: harvesting of the alga spirulina platens sunder zero-gravity conditions, Proceedings of World Congress on Ultrasonics, WCU 2003, Paris, France, September 7–10, 2003, pp. 1631–1638,

Benes E., Gröschl M., Radel S., Hauser C., Böhm H. (2005), New simple mathematical model for the separation performance of ultrasonic celllters, Proc. 2nd Congress of Alps – Adria Acoustics Association, 1st Congress of Acoustical Society of Croatia, June 23–24, 2005, Opatija, pp. 23–24.

Clement G.T. ( 2004), Perspectives in clinical uses of high-intensity focused ultrasound, Ultrasonics, 42, 10, 1087–1093, doi: 10.1016/j.ultras.2004.04.003.

Czyż H. (2003), Dispersed phase acoustics in liquid [in Polish], Name of Editorial Office, Rzeszów.

Gray H. (2008), Gray's anatomy: the anatomical basis of clinical practice, 40th ed., Elsevier, London.

Kozaczka E. (1988), Introduction to theory of non-linear hydroacoustics, Naval Academy Press, Gdynia.

Łętowska M. (2011), Medical principles of taking blood samples, separating blood components and distribution [in Polish], Institute of Hematology, Warszawa.

Nowicki A. (2010), Ultrasonics in medicine [in Polish], IPPT PAN, Warszawa.

Pashovkin T.N., Sadikova D.G. (2009), Cell exfoliation, separation, and concentration in the field of a standing ultrasonic wave, Acoustical Physics, 55, 4–5, 584–593,

Sadikova D.G., Andreev A.A., Shkidchenko A.N., Pashovkin T.N. (2006), Dynamics of cells concentration in a standing ultrasonic wave, Biomedical Technology and Electronics, 8–9, 95–99.

Śliwiński A. (2003), Ultrasonics and their application [in Polish], WNT, Warszawa.

Strippoli P. et al. (2013), An estimation of the number of cells in the human body, Annals of Human Biology, 40, 6, 463–471, doi: 10.3109/03014460.2013.807878.

Włoch A., Czyż H., Jasiński T. (2015), Ultrasonic methods of the cells separation in human blood, Acta Physica Polonica A, 128, 2, 234–236, doi: 10.12693/APhysPolA.128.234.

DOI: 10.24425/aoa.2019.128499