Archives of Acoustics, 39, 2, pp. 267-275, 2014

The Preparation of Soluble Cellouronic Acid Sodium Salt by 4-Acetamide-TEMPO Mediated Oxidation of Ultrasound-Pretreated Parenchyma Cellulose from Bagasse Pith

Kunming University of Science and Techonolgy

Kunming University of Science and Techonolgy

Kunming University of Science and Techonolgy

Lincai PENG
Kunming University of Science and Techonolgy

The parenchyma cellulose isolated from bagasse pith was used as an alternative resource for preparation of water-soluble cellouronic acid sodium salt (CAS). The influence of ultrasound treatment on the cellulose was investigated for obtaining CAS by regioselective oxidization using 4-acetamide-TEMPO and NaClO with NaClO$_2$ as a primary oxidant in an aqueous buffer at pH 6.0. The yield, carboxylate content and polymerization degree (DP) of CAS were measured as a function of ultrasonic power, agitating time and cellulose consistency by an orthogonal test. The ultrasound-treated conditions were further improved by discussion of ultrasonic power, the most important factor influencing the yield and DP. An optimized CAS yield of 72.9% with DP value (DPv) of 212 was found when the ultrasonic strength is 550 W, agitating time is 3 h and cellulose consistency is 2.0%. The oxidation reactivity of cellulose was improved by ultrasonic irradiation, whereas no significant changes in crystallinity of cellulose were measured after ultrasonic treatment. Moreover, the ultrasound treatment has a greater effect on yielding CAS from parenchyma cellulose than from bagasse fibrous’ one. The CAS was further characterized by Fourier
transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM).
Keywords: ultrasonic pretreatment; ultrasound power; bagasse pith; parenchyma cellulose; cellouronic acid sodium salt; 4-acetamide-TEMPO.
Full Text: PDF
Copyright © Polish Academy of Sciences & Institute of Fundamental Technological Research (IPPT PAN).


Biliuta G., Fras L., Drobota M., Persin Z., Kreze T., Kleinschek K.S., Ribitsch V., Harabagui V., Coseri S. (2013), Comparison study of TEMPO and phthalimide-N-oxy (PINO) radicals on oxidation efficiency toward cellulose, Carbohydrate Polymers, 91, 502-507.

Chen W.S., Yu H.P., Liu Y.X., Chen P., Zhang M.X., Hai Y.F. (2011a), Individualization of cellulose nanofibers from wood using high-intensity ultrasonication combined with chemical pretreatments, Carbohydrate Polymers, 83, 1804-1811.

Chen W. S., Yu H.P., Liu Y.X. (2011b), Preparation of millimeter- long cellulose I nanofibers with diameters of 30-80 nm from bamboo fibers, Carbohydrate polymers, 86, 453-461.

Coseri S., Biliuta G., Simionescu B.C., Kleinschek K.S., Ribitsch V., Harabagiu V. (2013), Oxidized cellulose-Survey of the most recent achievements, Carbohydrate Polymers, 93, 207-215.

Hirota M., Tamura N., Saito T., Isogai A. (2009), Oxidation of regenerated cellulose with NaClO2 catalyzed by TEMPO and NaClO under acid-neutral conditions, Carbohydrate Polymers, 78, 330-335.

Iwamoto S., Kai W H., Isogai T., Saito T., Isogai A., Iwata T. (2010), Comparison study of TEMPO-analogous compounds on oxidation efficiency of wood cellulose for preparation of cellulose nanofibrils, Polymer Degradation and Stability, 95, 1394-1398.

Jambrak A.R., Lelas V., Herceg Z., Badanjak M., Batur V., Muza M. (2009), Advantages and disadvantages of high power ultrasound application in the dairy industry, Mljekarstvo, 59, 4, 267-281.

Kumar V., Yang T.R. (2002), HNO3/H3PO4-NANO2 mediated oxidation of cellulose – preparation and characterization of bioabsorbable oxidized celluloses in high yields and with different levels of oxidation, Carbohydrate Polymers, 48, 403-412.

Liu C.F., Sun R.C., Qin M.H., Zhang A.P., Ren J.K., Ye J., Luo W., Cao Z.N. (2008), Succinoylation of sugarcane bagasse under ultrasound irraditation, Bioresource Technology, 99, 5, 1465-1473.

Reina T., Tsuguyuki S., Akira I. (2012), Cellulose nanofibrils prepared from softwood cellulose by TEMPO/NaClO/NaClO2 systems in water at pH 4.8 or 6.8, International Journal of Biological Macromolecules, 51, 228-234.

Ren Q.L. (2003), Optimization design and analysis of experiments [in Chinese: 试验优化设计与分析], Higher Education Press, Beijing, China.

Shibata I., Isogai A. (2003), Depolymerization of cellouronic acid during TEMPO-mediated oxidation, Cellulose, 10, 151-158.

Shinoda R., Saito T., Okita Y., Isogai A. (2012), Relationship between length and degree of polymerization of TEMPO-oxidized cellulose nanofibrils, Biomacromolecules, 13, 842-849.

Tang A., Zhang H., Chen G., Xie G.H., Liang W.Z. (2005), Influence of ultrasound treatment on accessibility and regioselective oxidation reactivity of cellulose, Ultrasonics Sonochemistry, 12, 467-472.

Yu H., Liu R.G., Shen D W., Wu Z.H., Huang Y. (2008), Arrangement of cellulose microfibrils in the wheat straw cell wall, Carbohydrate Polymers, 72, 122-127.

Vilkhu K., Mawson R., Simons L., Bates D. (2008), Applications and opportunities for ultrasound assisted extraction in the food industry—a review, Innovative Food Science and Emerging Technologies, 9, 2, 161-169.

Zhang K., Fischer S., Geissler A., Brendler E. (2012), Analysis of carboxylate groups in oxidized never-dried cellulose II catalyzed by TEMPO and 4-acetamide-TEMPO, Carbohydrate Polymers, 87, 894-900.

DOI: 10.2478/aoa-2014-0031