Archives of Acoustics, 9, 1-2, pp. 117-120, 1984

The passive haemagglutination reaction with sonicated t. Palliduni (nichols strain) in the serodiagnosis of syphilis (tpha)

Voichitza Lazar
Institute of Medicine and Pharmacy Babes-Bolyai University
Romania

Florica Kevorkian
Institute of Medicine and Pharmacy Babes-Bolyai University
Romania

Iuliu Capusan
Institute of Medicine and Pharmacy Babes-Bolyai University
Romania

Dezideriu Aus Lander
Institute of Medicine and Pharmacy Babes-Bolyai University
Romania

Eva Veress
Institute of Medicine and Pharmacy Babes-Bolyai University
Romania

The preparation of the antigen for TPHA sonicated suspension of T. pal. lidura (Nichols strain) being so far a "secret" of the procedures, we have attem¬pted to reproduce it by our own means of ultrasonic irradiation. The best results in agreement with the standard reactions for syphilis were obtained by sonicating T. pallidura suspensions for a duration of 20 min. by the following parameters of the ultrasonic field: frequency I MHz, intensity 0.9 W/cm2. Using this method we obtained microfragments of 1-1.3 microns. The most reliable dilution for the antigen was 1/1000. The haernagglutination was performed in 30 patients with different forms of late syphilis after treatment.
The results were in agreement with the clinical diagnosis, being more
sensitive and consistent compared with the classical and standard reactions.
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